sal1 hf (New England Biolabs)

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New England Biolabs sal1 hf
Sal1 Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sal1+hf/SalI-HF/pmc11190739-43-22-23
Average 98 stars, based on 520 article reviews

sal1 hf - by Bioz Stars, 2026-09

98/100 stars

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Purification:

Article Title: Discrimination between thermodynamic models of cis -regulation using transcription factor occupancy data
Article Snippet: The DNA was size-selected (150bp to 800bp) on a 1.5% TAE gel and purified, then ligated to annealed oligos RZ231 and RZ233 in the presence of EcoR1-HF (NEB R3101) and Mfe1 (NEB R0589S) and purified. .. The purified material was then ligated to annealed oligos RZ230 and RZ232 in the presence of Xho1 (NEB R0146S) and Sal1-HF (NEB R3138), then size selected (150-700 bp) on a 1.5% TAE Agarose gel . ..

Agarose Gel Electrophoresis:

Polymerase Chain Reaction:

Article Title: Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil
Article Snippet: The PCR products were purified using the MSC Spin 101 PCRapace kit (Stratec Molecular, Birkenfeld, Germany). .. The PCR products and p414GPD 102 expression vector (ATCC 87356) (22), which contains a resistance gene to ampicilin (AmpR) and 103 a tryptophan marker (TRP1), were digested using BamH1-HF and Sal1-HF (NEB, Ipswich, USA) 104 during 15 min at 37°C. .. The Lausannevirus DHFR-TS was ligated 1h at room temperature to the 105 p414GPD plasmid using a 3:1 ratio (insert:vector), the T4 DNA ligase and its buffer from Promega 106 (Dübendorf, Switzerland).

Article Title: Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil
Article Snippet: The PCR products were purified using the MSC Spin PCRapace kit (Stratec Molecular, Birkenfeld, Germany). .. The PCR products and the p414GPD expression vector (ATCC 87356) , which contains an ampicillin resistance ( ampR ) gene and a tryptophan marker (TRP1), were digested using BamH1-HF and Sal1-HF (NEB, Ipswich, MA, USA) for 15 min at 37°C. .. Lausannevirus DHFR-TS was ligated for 1 h at room temperature to the p414GPD plasmid at a 3:1 (insert/vector) ratio; the T4 DNA ligase and its buffer were from Promega (Dübendorf, Switzerland).

Expressing:

Article Title: Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil
Article Snippet: The PCR products were purified using the MSC Spin PCRapace kit (Stratec Molecular, Birkenfeld, Germany). .. The PCR products and the p414GPD expression vector (ATCC 87356) , which contains an ampicillin resistance ( ampR ) gene and a tryptophan marker (TRP1), were digested using BamH1-HF and Sal1-HF (NEB, Ipswich, MA, USA) for 15 min at 37°C. .. Lausannevirus DHFR-TS was ligated for 1 h at room temperature to the p414GPD plasmid at a 3:1 (insert/vector) ratio; the T4 DNA ligase and its buffer were from Promega (Dübendorf, Switzerland).

Plasmid Preparation:

Article Title: Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil
Article Snippet: The PCR products were purified using the MSC Spin PCRapace kit (Stratec Molecular, Birkenfeld, Germany). .. The PCR products and the p414GPD expression vector (ATCC 87356) , which contains an ampicillin resistance ( ampR ) gene and a tryptophan marker (TRP1), were digested using BamH1-HF and Sal1-HF (NEB, Ipswich, MA, USA) for 15 min at 37°C. .. Lausannevirus DHFR-TS was ligated for 1 h at room temperature to the p414GPD plasmid at a 3:1 (insert/vector) ratio; the T4 DNA ligase and its buffer were from Promega (Dübendorf, Switzerland).

Marker:

Article Title: Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil
Article Snippet: The PCR products were purified using the MSC Spin PCRapace kit (Stratec Molecular, Birkenfeld, Germany). .. The PCR products and the p414GPD expression vector (ATCC 87356) , which contains an ampicillin resistance ( ampR ) gene and a tryptophan marker (TRP1), were digested using BamH1-HF and Sal1-HF (NEB, Ipswich, MA, USA) for 15 min at 37°C. .. Lausannevirus DHFR-TS was ligated for 1 h at room temperature to the p414GPD plasmid at a 3:1 (insert/vector) ratio; the T4 DNA ligase and its buffer were from Promega (Dübendorf, Switzerland).

Review